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CHL/IU [CHL-11]
CHL/IU [CHL-11]
規格:
價格:
編號:B164247
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CHL/IU [CHL-11]
商品貨號
B164247
Organism
Cricetulus griseus, hamster, Chinese
Tissue
lung
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
newborn
Gender
female
Applications
The line is useful for screening chemical for ability to induce chromosomal aberrations.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 25
Derivation
This line was derived from the CHL line established by Utakoji in 1970.
Complete Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; bovine calf serum, 10%
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
Complete growth medium supplemented with 5% DMSO
Culture Conditions
Temperature: 37°C
Population Doubling Time
15 hrs
Name of Depositor
M Ishidate
Deposited As
Cricetulus griseus
Year of Origin
1970
References

Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988.

Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270

Koyama H, et al. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405

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