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CKMM 14.15
CKMM 14.15
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164261
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CKMM 14.15
商品貨號(hào)
B164261
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen 1.0 mL
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to both CK-MM and CK-MB.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CK-MM and CK-MB (creatine kinase - MM and MB)
Cellular Products
immunoglobulin; monoclonal antibody; against human CK-MM and CK-MB (creatine kinase - MM and MB)
Comments
Animals were immunized with human creatine kinase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to both CK-MM and CK-MB.
Binding of the antibody inhibits the enzyme activity.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose and 1.0 mM sodium pyruvate, 95%; fetal bovine serum, 5%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Volume
1.0 mL
Isotype
IgG2a; kappa light chain
Name of Depositor
Eastman-Kodak Co.
Deposited As
rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Daiss JL. Blend of monoclonal antibodies. US Patent 4,950,592 dated Aug 21 1990

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