Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224).
The cells are CSAp negative (CSAp-).
COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
Cells grow loosely attached and in suspension.
Shake flask, retain the floating cells by transfering them into a centrifuge tube. Cells that remain attached may be removed using a standard trypsinization protocol (see below) and combined with the retained floating cells. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251
Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697
Nucleotide (GenBank) : AB012142 Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds.
Nucleotide (GenBank) : AB012143 Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds.
