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COLO 320DM
COLO 320DM
規格:
價格:
編號:B164284
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
COLO 320DM
商品貨號
B164284
Organism
Homo sapiens, human
Tissue
colon
培養基
Product Format
frozen
Morphology
rounded and refractile
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Dukes' type C, colorectal adenocarcinoma
Age
55 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 24; range = 49 to 61.
The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.
Images
Clinical Data
55 years
Caucasian
female
Genes Expressed
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Cellular Products
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments
The line is negative for CEA, CSAp and colon antigen 3.
The cells are weakly positive for keratins and vimentin.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.
  1. Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
  2. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  3. Add appropriate aliquots of the cell suspension to new culture vessels.
  4. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 9
TPOX: 8,9
vWA: 15,18

Note: This cell line has historically exhibited instability at D7S820 # 9 and TH01 # 8

Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Name of Depositor
GE Moore
Deposited As
Homo sapiens
References

Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

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