Comments
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.
The cells produce very high levels of alpha and beta hCG.
The line contains the plasmid pRF 398 alpha t2.
The construct contains the entire sequence of beta hCG plus 30 bp 5' untranslated and 60 bp 3' untranslated under the control of the mouse metallothionein promoter.
The construct contains the entire sequence of alpha hCG plus 10 bp 5' untranslated and 220 bp 3' untranslated under the control of the SV40 early promoter.
An EcoRI/BamHI fragment of palphat2 (containing alpha hCG and SV40 sequences) was inserted in the BamHI site of pRF398 (ATCC CRL 8401) (containing beta hCG, MT-1 promoter, and BPV sequences) to create pRF398alphat2.
