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CW13.20-3B3 (clone of BCL1)

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編號:B164307

品牌:Mingzhoubio

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產品名稱

CW13.20-3B3 (clone of BCL1)

商品貨號

B164307

Organism

Mus musculus, mouse

Tissue

peripheral blood

培養基

Cell Type

B lymphocyte

Product Format

frozen

Morphology

clusters of lymphoblast-like cells and single attached cells with a dendritic-like appearance

Culture Properties

Mixed: adherent and suspended

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

leukemia

Strain

BALB/c

Storage Conditions

liquid nitrogen vapor phase

Antigen Expression

H-2d

Genes Expressed

immunoglobulin

Comments

This line does not produce IgM unless stimulated with B Cell Differentiation Factor (BCDF, interleukin-6, interleukin 6, IL-6), however some lots of fetal bovine serum contain levels of endotoxin which may induce a background level of IgM secretion. In order for cells to function optimally in the BCDF assay using IgM secretion, it is essential to pretest fetal bovine serum (FBS) and select lots that are low in endotoxins and do not induce high background levels of IgM secretion. Background secretion should be less than 400 ng/mL.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol

Subculturing

The cells loosely adhere to the substrate, and subcultures are prepared when the culture is 75% to 90% confluent by striking the flask sharply 3 to 4 times against the palm of the hand. Free floating cells, which accumulate with time in culture, are viable and can be used to initiate new cultures.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Isotype

IgM

Name of Depositor

K Brooks, E Vitetta

Deposited As

Mus musculus

References

Brooks K, et al. Lymphokine-induced IgM secretion by clones of neoplastic B cells. Nature 302: 825-826, 1983. PubMed: 6601774

Cross References

Nucleotide (GenBank) : U08185 Mus musculus BALB/c zinc-finger protein Blimp-1 mRNA, complete cds.

Nucleotide (GenBank) : NM_007548 Mus musculus PR domain containing 1, with ZNF domain (Prdm1), mRNA.

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