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D10.G4.1
D10.G4.1
規格:
價格:
編號:B164312
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D10.G4.1
商品貨號
B164312
Organism
Mus musculus, mouse
Tissue
lymph node
培養基
Cell Type
helper T lymphocyte (Th-2)
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
adult
Gender
male
Strain
AKR/J
Applications
D10.G4.1 has been used extensively to study activation of T cells. These cells can also be used as a reliable and sensitive assay for IL-1 (interleukin-1) response, since D10 cells will proliferate only minimally to con A in the absence of IL-1 or feeder cells.
Storage Conditions
liquid nitrogen vapor phase
Images
Clinical Data
male
Antigen Expression
H-2k
Receptor Expression

interleukin 1 (IL-1), expressed; interleukin 1 (IL-1)

Genes Expressed
interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
Cellular Products
interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
Comments
The cells are specific for egg white conalbumin (Sigma No. C0755) and alloreactive to I-A b, v.
Supernatant media from Con A stimulated D10.G4.1 cells contains IL-4 and IL-5.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 10% T-STIM with Con A (rat IL-2 culture supplement ) Becton Dickinson, Catalog No. 354115)
  • 10% fetal bovine serum
  • 0.05 mM 2-mercaptoethanol
  • 10 pg/ml mouse IL-1 alpha (R&D Systems, Catalog No. 400ML)

Subculturing
Subculture actively growing suspension cultures before they have reached 1 X 106 cells/mL or the IL-1 and IL-2 will be rapidly depleted and the cells will lose viability. Use inoculation densities of 2 X 105 viable cells/mL.
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 5% (v/v) DMSO.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
C Janeway, J Kaye, P Conrad
Deposited As
Mus musculus
References

Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584

Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236

Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337

Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287

Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148

Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572

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