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DA4-4
DA4-4
規格:
價格:
編號:B164325
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DA4-4
商品貨號
B164325
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human mu heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human mu heavy chain
Comments
Animals were immunized with human IgM.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 92.5%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
JF Kearney, H Kubagawa
Deposited As
mouse (B cell); mouse (myeloma)
References

Maruyama S, et al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. J. Immunol. 135: 192-199, 1985. PubMed: 3923101

Nahm MH, et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. PubMed: 9291318

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