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DAKIKI
DAKIKI
規格:
價格:
編號:B164326
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DAKIKI
商品貨號
B164326
Organism
Homo sapiens, human
Cell Type
lymphoblast; Epstein-Barr virus (EBV) transforme
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Clinical Data
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV.
Genes Expressed
immunoglobulin; monoclonal antibody
Cellular Products
immunoglobulin; monoclonal antibody
Comments
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgA; lambda light chain
Name of Depositor
P Ralph
Deposited As
Homo sapiens
References

Steinitz M, Klein G. EBV-transformation of surface IgA-positive human lymphocytes. J. Immunol. 125: 194-196, 1980. PubMed: 6247394

Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913

Cross References

Nucleotide (GenBank) : U07986 Human immunoglobulin IgH heavy chain Fd fragment mRNA, partial cds.

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