最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > DAN B164330
DAN
DAN
規格:
價格:
編號:B164330
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DAN
商品貨號
B164330
Organism
Canis familiaris, dog
Tissue
bone
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
11 years
Gender
female
Strain
poodle
Applications
DAN cells were isolated after selection with G418 and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Storage Conditions
liquid nitrogen vapor phase
Derivation
DAN cells were isolated after selection with G418 and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Clinical Data
female
Comments
DAN is a retrovirus packaging cell line derive from the D-17 canine osteogenic sarcoma cell line (ATCC CCL-183) by Howard Temin.
D-17 cells were transfected with plasmids pBR1 (gag - pol genes from spleen necrosis virus), pJD1 (env gene from amphotropic murine leukemia virus) and pSV2neo (G418 resistance).
DAN cells were isolated after selection with G418 and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Complete Growth Medium
Minimum essential medium (Eagle) with Earle's BSS containing 0.4 mg/ml G418, 92%; fetal bovine serum, 8%
Subculturing
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Inoculate new flasks with 1 to 2 X 10(5) cells per sq cm.
Interval: Subculture at or prior to becoming confluent.
Medium Renewal: Twice per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor
H Temin, DW Burns
Deposited As
Canis familiaris
References

Dougherty JP, et al. New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors. J. Virol. 63: 3209-3212, 1989. PubMed: 2524600

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479