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DDT1 MF-2
DDT1 MF-2
規(guī)格:
價(jià)格:
編號(hào):B164340
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
DDT1 MF-2
商品貨號(hào)
B164340
Organism
Mesocricetus auratus, hamster, Syrian golden
Tissue
ductus deferens; smooth muscle
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
leiomyosarcoma
Gender
male
Applications
transfection host
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
male
Receptor Expression
androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Comments
Growth of DDT1 MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor
JS Norris
Deposited As
Mesocricetus auratus
References

Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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