Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
This cell line can be transformed by SV40.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
Miyatake S, et al. Transcriptional targeting of herpes simplex virus for cell-specific replication. J. Virol. 71: 5124-5132, 1997. PubMed: 9188579
