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DU4475
DU4475
規格:
價格:
編號:B164380
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DU4475
商品貨號
B164380
Organism
Homo sapiens, human
Tissue
mammary gland; breast; derived from metastatic site: skin carcinoma
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma, skin
Age
70 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 93; range = 85 to 95.
The cell line is aneuploid human female, with chromosome counts in the tetraploid range. Normal chromosomes N16 and N21 are least well represented, with from none to two copies per karyotype, while chromosome N7 tends to be over-represented, with four or five copies per karyotype, whereas two to four copies are found of most of the remaining normal chromosomes. Twenty-two marker chromosomes are found including: iso(1q), del(1)(q32), der(1)t(1;?)(p36;?), del(3)(q24), der(3)t(3;?)(q24;?), del(3)(p14p25), t(6q;21?) and others. Eight are identical to those reported or portrayed in the report by A.J. Langlois, et al.
Images
Clinical Data
70 years
Caucasian
female
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments
Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy.
This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests.
The cells form transplantable tumors similar in histology to that of the original carcinoma.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,12
D13S317: 11,14
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 6,8
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1-2
PGM3, 1
Name of Depositor
AJ Langlois
Deposited As
Homo sapiens
References

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108

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