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E13 161-7
E13 161-7
規(guī)格:
價格:
編號:B164387
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
E13 161-7
商品貨號
B164387
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an antigen expressed on mouse hematopoietic stem cells.
Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63 myeloma cells.
Genes Expressed
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus.
immunoglobulin; monoclonal antibody; against mouse stem cell antigen 1 (Sca-1),Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells.
Cellular Products
immunoglobulin; monoclonal antibody; against mouse stem cell antigen 1 (Sca-1)
Comments
Animals were immunized with a pre-T cell hybridoma.
Spleen cells were fused with P3X63 myeloma cells.
The antibody reacts with an antigen expressed on mouse hematopoietic stem cells.
Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a , IgG2a
Name of Depositor
IL Weissman
Deposited As
rat (B cell); mouse (myeloma)
References

Aihara Y, et al. An attempt to produce ``pre-T'' cell hybridomas and to identify their antigens. Eur. J. Immunol. 16: 1391-1399, 1986. PubMed: 3096743

Spangrude GJ, et al. Purification and characterization of mouse hematopoietic stem cells. Science 241: 58-62, 1988. PubMed: 2898810

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