Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131
The EGC/PK060399egfr enteroglial cell line was established on March 6, 1999 from adult rat myenteric plexus from the jejunum. Purified primaries were transfected with supernants from the virus producing cell line GPE-egfr-neu. GPE-egfr-neu cells contain the chimeric gene egfr/neu and the neomycin-resistance gene neo under the control of the long-terminal repeats of the mouse Moloney leukaemia virus. The egfr/neu gene codes for the extracellular domain of the human EGF receptor and the intracellular tyrosine kinase domain of the proto-oncogene c-neu.
