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EIAV 12E8.1
EIAV 12E8.1
規格:
價格:
編號:B164399
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
EIAV 12E8.1
商品貨號
B164399
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)
Cellular Products
immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)
Comments
Animals were immunized with p26 prepared from the Wyoming strain of EIA virus.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
DMEM, 70%; fetal bovine serum, 20%; DMSO, 10%
Isotype
IgG1
Name of Depositor
Fermenta Animal Health Company
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. US Patent 4,806,467 dated Feb 21 1989

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