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EL4.IL-2
EL4.IL-2
規格:
價格:
編號:B164407
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
EL4.IL-2
商品貨號
B164407
Organism
Mus musculus, mouse
Tissue
thymus
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
C57BL/6
Storage Conditions
liquid nitrogen vapor phase
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).
The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL. Maintain cell density between 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor
JJ Farrar
Deposited As
Mus musculus
References

Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

Cancer therapeutics. New York: Raven Press; 1981.

Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931

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