Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. Ref
Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cells exhibit phagocytic activity.
These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens but unlike EOC-20 (CRL-2469) expression was not upregulated by recombinant murine interferon-gamma.
C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Subculturing Procedure for LADMAC cells
Cultures can be established by centrifugation with subsequent resuspension at 2 X 10 5 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Complete Growth Medium for LADMAC cells
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.? To make the complete growth medium, add the following components to the base medium:?
This medium is formulated for use with a 5% CO2 in air atmosphere.
Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530
Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775
