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F1-3G8-1
F1-3G8-1
規格:
價格:
編號:B164422
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
F1-3G8-1
商品貨號
B164422
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the F1 antigen of plague bacillus and may be of use as a diagnostic reagent.
The antibody has been used to establish an ELISA method for use in quantitating the F1 antigen.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bubonic plague bacillus; Yersinia pestis (F1 antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against bubonic plague bacillus; Yersinia pestis (F1 antigen)
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with plague bacillus.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody is specific for the F1 antigen of plague bacillus and may be of use as a diagnostic reagent.
The antibody has been used to establish an ELISA method for use in quantitating the F1 antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype
    IgA
    Name of Depositor
    MK Gentry, JE Williams
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Gentry MK, et al. Use of an enzyme-linked immunosorbent assay to measure antigenaemia during acute plague. Bull. World Health Organ. 62: 463-466, 1984. PubMed: 6380787

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