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F18 AF1
F18 AF1
規格:
價格:
編號:B164423
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
F18 AF1
商品貨號
B164423
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420).
Genes Expressed
immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I)
Cellular Products
immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420).
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Amgen, Inc.
U.S. Patent Number
References

Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985

. . Hybridoma 2: 137, 1983.

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