Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas.
The F19 antigen is not detected on SV40 transformed fibroblasts.
During scar formation, the antigen is expressed in the scar but not in the surrounding tissue.
The F19 antigen is also often expressed in the normal stroma surrounding the growth of tumors.
A culture deposited with the ATCC as HB-8269 in March of 1983 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as ATCC CRL-2733. The original patent deposit is available as HB-8269.
Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991
Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
