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F3B6

規(guī)格:

價格:

編號:B164428

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

F3B6

商品貨號

B164428

Organism

human (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The cells are resistant to 6-thioguanine.

This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.

Comments

Fusion of human peripheral blood B cell with NS-1 myeloma cells.

The cells are resistant to 6-thioguanine.

This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.

Complete Growth Medium

Culture Medium: HL-1 medium, 99%; fetal bovine serum, 1%. Iscove's modified
Dulbecco's medium with 10% fetal bovine serum has been used as an alternative
to HL-1.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 1 X 10 exp6 cells/ml.

Cryopreservation

HL-1 medium, 82.5%, human serum albumin, 10%; DMSO, 7.5%

Name of Depositor

Cetus Corp.

Deposited As

human (B cell); mouse (myeloma)

U.S. Patent Number

4,677,070

References

Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987

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