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FAK-/-
FAK-/-
規(guī)格:
價格:
編號:B164434
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
FAK-/-
商品貨號
B164434
Organism
Mus musculus, mouse
Tissue
embryo
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
8 days gestation
Applications
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
The control cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Expression of the FAK-related protein-tyrosine kinase, Pyk2, is elevated in FAK-/- p53-/- fibroblasts compared with FAK+/+ p53-/- cells.
Derivation
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
The control cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene. Therefore the cells are genetically FAK+/+ p53-/-.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Clinical Data
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Oncogene
p53 -
Genes Expressed
p53 -
Comments
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
Therefore, the cells are genetically FAK-/- p53-/-.
The control cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene. Therefore the cells are genetically FAK+/+ p53-/-.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Expression of the FAK-related protein-tyrosine kinase, Pyk2, is elevated in FAK-/- p53-/- fibroblasts compared with FAK+/+ p53-/- cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Population Doubling Time
12 hrs
Name of Depositor
D Ilic
Deposited As
mouse
References

Ilic D, et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377: 539-544, 1995. PubMed: 7566154

Sieg DJ, et al. Pyk2 and Src-family protein-tyrosine kinases compensate for the loss of FAK in fibronectin-stimulated signaling events but Pyk2 does not fully function to enhance FAK- cell migration. EMBO J. 17: 5933-5947, 1998. PubMed: 9774338

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