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FB2
FB2
規(guī)格:
價格:
編號:B164438
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
FB2
商品貨號
B164438
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions.
Derivation
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against phosphotyrosine
Cellular Products
immunoglobulin; monoclonal antibody; against phosphotyrosine
Comments
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions.
Thymidine and aminopterin are added to the culture medium to stabilize the presence of the immunoglobulin heavy chain locus.
Complete Growth Medium
RPMI 1640 medium with 10 mM HEPES, 0.0039 mg/ml thymidine, 176 ng/ml aminopterin and 0.075 mM adenine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG3; kappa light chain
Name of Depositor
J Burkhart, LT Williams
Deposited As
mouse (B cell); mouse (myeloma)
References

Frackelton AR Jr., et al. Evidence for the platelet-derived growth factor-stimulated tyrosine phosphorylation of the platelet-derived growth factor receptor in vivo. Immunopurification using a monoclonal antibody to phosphotyrosine. J. Biol. Chem. 259: 7909-7915, 1984. PubMed: 6203898

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