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當(dāng)前位置: 首頁(yè) > ATCC代理 > FHCR-1-2813/FDC-6 B164456
FHCR-1-2813/FDC-6
FHCR-1-2813/FDC-6
規(guī)格:
價(jià)格:
編號(hào):B164456
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
FHCR-1-2813/FDC-6
商品貨號(hào)
B164456
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Hepatoma
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with human fibronectin from fetal and neoplastic tissues, but not from normal human tissues or plasma.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with SP-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an onco-fetal determinant on human fibronectin
Cellular Products
immunoglobulin; monoclonal antibody; against an onco-fetal determinant on human fibronectin
Comments
Animals were immunized with fibronectin from HuH-6 human hepatoma cells.
Spleen cells were fused with SP-1 myeloma cells.
The antibody reacts with human fibronectin from fetal and neoplastic tissues, but not from normal human tissues or plasma.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 88%; fetal bovine serum, 12%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Fred Hutchinson Cancer Res. Cntr.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Loridon-Rosa B, et al. Distribution of oncofetal fibronectin in human mammary tumors: Immunofluorescence study on histological sections. Cancer Res. 50: 1608-1612, 1990. PubMed: 2406016

Matsuura H, Hakomori S. The oncofetal domain of fibronectin defined by monoclonal antibody FDC-6: Its presence in fibronectins from fetal and tumor tissues and its absence in those from normal adult tissues and plasma. Proc. Natl. Acad. Sci. USA 82: 6517-6521, 1985. PubMed: 2995969

Matsuura H, et al. The oncofetal structure of human fibronectin defined by monoclonal antibody FDC-6. Unique structural requirement for the antigenic specificity provided by a glycosylhexapeptide. J. Biol. Chem. 263: 3314-3322, 1988. PubMed: 2449438

Matsuura H, Hakomori S. Monoclonal antibody defining oncofetal structure of fibronectin. US Patent 4,894,326 dated Jan 16 1990

Matsuura H, et al. An alpha-N-acetylgalactosaminylation at the threonine residue of a defined peptide sequence creates the oncofetal peptide epitope in human fibronectin. J. Biol. Chem. 264: 10472-10476, 1989. PubMed: 2471705

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