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FHs 74 Int
FHs 74 Int
規格:
價格:
編號:B164460
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
FHs 74 Int
商品貨號
B164460
Organism
Homo sapiens, human
Tissue
small intestine
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
3 to 4 months gestation
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Karyotype
normal female; diploid
Images
Clinical Data
female
Receptor Expression
epidermal growth factor (EGF), expressed
Tumorigenic
No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
PAS, negative.
The cells are negative for keratin by immunoperoxidase staining.
Complete Growth Medium
Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
(Do not filter the medium after addition of EGF)
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage Temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,11
    D13S317: 11,12
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 9,10
    THO1: 7,9.3
    TPOX: 8
    vWA: 16,18
    Isoenzymes
    AK-1, 1
    ES-D, 1-2
    G6PD, B
    GLO-I, 1
    Me-2, 1-2
    PGM1, 1
    PGM3, 1
    Name of Depositor
    R Owens
    Deposited As
    Homo sapiens
    References

    Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

    Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

    Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205

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