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當(dāng)前位置: 首頁(yè) > ATCC代理 > FW11-9-2 B164474
FW11-9-2
FW11-9-2
規(guī)格:
價(jià)格:
編號(hào):B164474
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
FW11-9-2
商品貨號(hào)
B164474
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v).
The antibody is specific for the product of the 6v exon.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the v6 variant of human CD44
Cellular Products
immunoglobulin; monoclonal antibody; against the v6 variant of human CD44
Comments
Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the product of the 6v exon.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
CR Mackay
Deposited As
mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492

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