Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Gardner's syndrome
Age
48 years
Gender
female
Ethnicity
Caucasian
Derivation
The cells are said to exist in an initiated state as opposed to a normal or transformed state.
Clinical Data
The tissue donor suffered from Gardner's syndrome.
female
Caucasian
48 years
Comments
The tissue donor suffered from Gardner's syndrome.
The cells are said to exist in an initiated state as opposed to a normal or transformed state.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
