色伊人久久,国产高清不卡,久久久999成人

H19-7/IGF-IR

規格:

價格:

編號:B164525

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

H19-7/IGF-IR

商品貨號

B164525

Organism

Rattus norvegicus, rat

Tissue

brain/hippocampus

培養基

Cell Type

fibroblast

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

2 [Cells contain SV40 DNA Viral Sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

embryo; 17 day gestation

Strain

Holtzman

Applications

This cell line can be used for analysis and comparison of mitogenesis (34°C) and differentiation (39°C).

Storage Conditions

liquid nitrogen vapor phase

Derivation

The H19-7 cell line was derived from hippocampi dissected from embryonic day 17 (E17) Holtzman rat embryos and immortalized by retroviral transduction of temperature sensitive tsA58 SV40 large T antigen. H19-7/IGF-IR cells were established by infecting H19-7 cells with a retroviral vector expressing the human type I insulin-like growth factor receptor (IGF-IR). The cells were selected in medium containing puromycin.

Receptor Expression

human type I insulin-like growth factor receptor (IGF-IR)

Comments

H19-7 cells grow at the permissive temperature (34°C) in epidermal growth factor or serum.

They differentiate to a neuronal phenotype at the nonpermissive temperature (39°C) when induced by basic fibroblast growth factor (bFGF) in N2 medium (DMEM-high glucose medium with supplements).

At 39°C, expression of the human IGF-IR in H19-7 cells induces an insulin-like growth factor (IGF) I dependent differentiation. The cells extend neurites and show increased expression of NF68.

H19-7/IGF-IR cells express the IGF-IR protein. IGF-IR is known to send two seemingly contradictory signals inducing either cell proliferation or cell differentiation, depending on cell type and/or conditions.

This cell line does not express detectable levels of the SV40 T antigen.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.2 mg/ml G418

0.001 mg/ml puromycin

fetal bovine serum to a final concentration of 10%

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Note: Flasks used to propagate these cells must be coated with 0.015 mg/mL poly-l-lysine. Cover the surface of the vessel with poly-l-lysine solution for 5 minutes, remove, and let dry.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 34°C.

Subculture Ratio: 1:3 to 1:5

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 34°C

Name of Depositor

R Baserga, A Morrione

Deposited As

rat

References

Morrione A, et al. Insulin-like growth factor I receptor signaling in differentiation of neuronal H19-7 cells. Cancer Res. 60: 2263-2272, 2000. PubMed: 10786694

Eves EM, et al. Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. Proc. Natl. Acad. Sci. USA 89: 4373-4377, 1992. PubMed: 1316607

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479

<dfn id="rdt2g"></dfn>