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H25B10
H25B10
規格:
價格:
編號:B164531
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
H25B10
商品貨號
B164531
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatitis
Applications
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Cellular Products
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Comments
Animals were immunized with hepatitis B virus surface antigen.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Massachusetts General Hospital
Deposited As
mouse (B cell); mouse (myeloma)
References

Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981

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