Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323.
HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of Her2-neu; they overexpress p53.
The cells are negative for expression of progesterone receptor (PR).
Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. Cultures can be maintained by the addition of fresh medium to the flask, or centrifuging and removing the spent medium, resuspending the cell pellet in fresh medium and returning the resuspended cells to the flask.
To subculture the attached cells, remove and reserve the suspended cells (recover the suspended cells by centrifugation if desired). Rinse the monolayer with fresh 0.25% trypsin-0.53 mM EDTA, neutralize with an equal volume complete medium and centrifuge. Remove supernatant and resuspend cell pellet with complete medium and pool into reserved suspended cells. Add appropriate aliquots of the pooled cell suspension to new culture vessels.
Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
