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HCC1428

規(guī)格:

價格:

編號:B164558

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

HCC1428

商品貨號

B164558

Organism

Homo sapiens, human

Tissue

mammary gland/breast; derived from metastatic site: adenocarcinoma and pleural effusion

培養(yǎng)基

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent; large epithelial cells with occasional vacuole formation

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

TNM stage IV, grade 4, adenocarcinoma

Age

49 years

Gender

female

Ethnicity

Caucasian, White

Storage Conditions

liquid nitrogen vapor phase

Karyotype

polyploid

Derivation

HCC1428 was derived from a female who harbored a homozygous deletion in the FHIT gene.

This cell line was initiated on 11/4/95 and took 15 months to establish.

Clinical Data

female

Caucasian, White

49 years

There is a family history of breast cancer (maternal grandmother).

Oncogene

her2/neu -, p53 -

Genes Expressed

HCC1428 cells are positive for the expression of Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

The cells are negative for expression of Her2-neu and for expression of p53.

Comments

An EBV transformed lymphoblastoid cell line [HCC1428 BL] from the same patient is available as ATCC CRL-2328. The homozygous deletion in the FHIT gene is retained in the lymphoblastoid line.

The FHIT gene, which spans the FRA3B fragile site at chromosome 3p14.2, is a candidate tumor suppressor gene in breast and other cancers.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:4 to 1:8

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

CSF1PO: 10

D13S317: 12

D16S539: 12,8

D5S818: 11,12

D7S820: 9

THO1: 8

TPOX: 8

vWA: 17

Name of Depositor

AF Gazdar, AK Virmani

Year of Origin

November 4, 1995

References

Ahmadian M, et al. Analysis of the FHIT gene and FRA3B region in sporadic breast cancer, preneoplastic lesions, and familial breast cancer probands. Cancer Res. 57: 3664-3668, 1997. PubMed: 9288768

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

B164559：HCC1428 BL

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