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HCC1599
HCC1599
規格:
價格:
編號:B164562
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HCC1599
商品貨號
B164562
Organism
Homo sapiens, human
Tissue
mammary gland; breast/duct
培養基
Cell Type
epithelial, lymphoblast
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
TNM stage IIIA, grade 3, primary ductal carcinoma
Age
44 years adult
Gender
female
Ethnicity
Caucasian, White
Applications
HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
The cells are negative for expression of Her2-neu and for expression of p53.
The cells are poorly differentiated.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
multiploid. The cell population has several ploidy indices.
Images
Cell Micrograph ATCC CRL-2331, HCC1599
Derivation
The HCC1599 breast cancer cell line was initiated on March 28, 1995, and took 10 months to establish.
Clinical Data
44 years
An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.
Caucasian, White
female
Receptor Expression
estrogen receptor, negative
progesterone receptor, negative
Oncogene
her2/neu -, p53 -
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments
The tumor was classified as a TNM stage IIIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.
The cells are poorly differentiated.
The cells are negative for expression of Her2-neu and for expression of p53.
The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.
An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 12,13
D5S818: 12
D7S820: 10,11
THO1: 9.3
TPOX: 8
vWA: 17
Name of Depositor
AF Gazdar, AK Virmani
Year of Origin
March 28, 1998
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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