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HCC1937 BL

規(guī)格:

價(jià)格:

編號(hào):B164567

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

HCC1937 BL

商品貨號(hào)

B164567

Organism

Homo sapiens, human

Tissue

peripheral blood

培養(yǎng)基

Cell Type

B lymphoblast; Epstein-Barr virus (EBV) transformed

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension, multicell aggregates

Biosafety Level

2 [Cells contain Epstein Barr Virus (EBV)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

24 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

HCC1937BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC1937 (a primary breast ductal carcinoma cell line, ATCC CRL-2336).

Oncogene

BRCA1 (mutated, insertion C at nucleotide 5382)

Comments

BRCA1 analysis revealed that this cell line is heterozygous for the BRCA1 5382C mutation, whereas the primary tumor cell line is homozygous for the same mutation.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 10⁵ viable cells/mL. Maintain cultures at cell concentrations between 5 x 10⁵ and 2 x 10⁶ viable cells/mL.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).

Cryopreservation

Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

Name of Depositor

AF Gazdar, AK Virmani

References

Tomlinson GE, et al. Characterization of a breast cancer cell line derived from a germ-line BRCA1 mutation. Cancer Res. 58: 3237-3242, 1998. PubMed: 9699648

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

B239830：Human genomic DNA (HCC1937 BL)

B233310：Human Total RNA (HCC1937)

B209829：Human genomic DNA (HCC1937)

B164566：HCC1937

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