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HCC202

規(guī)格:

價(jià)格:

編號(hào):B164570

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

HCC202

商品貨號(hào)

B164570

Organism

Homo sapiens, human

Tissue

breast; mammary gland/duct

培養(yǎng)基

Cell Type

Epithelial

Product Format

frozen

Culture Properties

adherent, The line grows as attached medium-sized epithelial cells with some floating cells.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

TNM stage IIIA, grade 3, primary ductal carcinoma

Age

82 years

Gender

female

Ethnicity

Caucasian, White

Karyotype

polyploid

Derivation

The HCC202 cell line was initiated from a primary ductal carcinoma on September 5, 1992, and took 41 months to establish.

Clinical Data

82 years

Caucasian, White

female

Receptor Expression

estrogen receptor, negative

progesterone receptor, negative

Oncogene

her2/neu +, p53 -

Genes Expressed

Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

Cellular Products

Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

Comments

The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 19 lymph nodes.

The cells are poorly differentiated.

The cells are positive for expression of Her2-neu, but are negative for expression of p53.

HCC202 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are negative for expression of estrogen receptor (ER -) and progesterone receptor (PR -).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:2 to 1:3

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10

D13S317: 8,9

D16S539: 13

D5S818: 11,13

D7S820: 8,12

THO1: 6

TPOX: 8,9

vWA: 16

Name of Depositor

AF Gazdar, AK Virmani

Deposited As

Homo sapiens

Year of Origin

September 5, 1992

References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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