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HCC70
HCC70
規格:
價格:
編號:B164576
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HCC70
商品貨號
B164576
Organism
Homo sapiens, human
Tissue
mammary gland; breast/duct
培養基
Cell Type
Epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent, The line grows as attached medium-sized epithelial cells without floating cells.
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
TNM stage IIIA, grade 3,primary ductal carcinoma
Age
49 years
Gender
female
Ethnicity
Black
Applications
HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells.
The cells are poorly differentiated.
The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.
The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
polyploid
Images
ATCC CRL-2315 Micrograph
Derivation
The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
Clinical Data
49 years
Black
female
Receptor Expression
progesterone receptor, not expressed
Oncogene
her2/neu -, p53 + (overexpressed)
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19,her2/neu -, p53 + (overexpressed),The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.,The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments
The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
The cells are poorly differentiated.
The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes.
The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.
HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. An independent study, by ATCC Scientists, is underway to assess the estrogen receptor status; results of this study will be published online upon completion.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin:X
CSF1PO:10,14
D13S317:12
D16S539:9,13
D5S818:12,13
D7S820:10,11
THO1:9
TPOX:10
vWA: 13,15
Name of Depositor
AF Gazdar, AK Virmani
Deposited As
Homo sapiens
Year of Origin
1992
References

Kao J, et al. Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery. PlosONE 4 (7): e 6146, 2009

Kim MS, et al. Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform. PlosONE 5 (5): e10441, 2010

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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