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Hybridoma, hCD40L-M90 [ hCD40L-M90, M90]
Hybridoma, hCD40L-M90 [ hCD40L-M90, M90]
規格:
價格:
編號:B164577
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hybridoma, hCD40L-M90 [ hCD40L-M90, M90]
商品貨號
B164577
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD40 ligand (CD154, CD40L)
Cellular Products
immunoglobulin; monoclonal antibody; against human CD40 ligand (CD154, CD40L)
Comments
Animals were immunized with human CD40 ligand (CD154, CD40L).
Spleen cells were fused with either Ag8.653 or NS1 mouse myeloma cells (the specific line used was not specified).
The antibody recognizes the human CD40L polypeptide.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Immunex Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Armitage RJ, et al. Monoclonal antibodies to CD40 ligand, pharmaceutical composition comprising the same and hybridomas producing the same. US Patent 5,961,974 dated Oct 5 1999

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