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HECA-452
HECA-452
規格:
價格:
編號:B164588
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HECA-452
商品貨號
B164588
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: Wistar

Applications
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Cellular Products
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA)
Comments
The antibody reacts with an antigen on skin homing memory T cells.
It binds a T cell carbohydrate ligand for vascular E-selectin.
The antibody also recognizes sialyl Lewis x and sialyl Lewis a antigens and other carbohydrate ligands for human E-selectin.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and non essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
rat IgM
Name of Depositor
Stanford University
Deposited As
rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994

Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207

Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810

The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.

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