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HGH-B

規格:

價格:

編號:B164607

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HGH-B

商品貨號

B164607

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with mouse myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human growth hormone (hGH)

Cellular Products

immunoglobulin; monoclonal antibody; against human growth hormone (hGH)

Tumorigenic

Yes

Effects

Yes, forms ascites in BALB/c mice

Comments

Animals were immunized with hGH.

Spleen cells were fused with mouse myeloma cells.

Complete Growth Medium

Culture Medium: Dulbecco's modified Eagle's medium (containing: 2 mM
L-glutamine and 1 mM sodium pyruvate) with 4.5 g/L glucose, 90%; fetal bovine
serum, 10%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

Culture medium, 95%; DMSO, 5%

Isotype

IgG1; kappa light chain

Name of Depositor

Genentech, Inc.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

5,210,017

References

Carlsson LM, et al. Ligand-mediated immunofunctional hormone binding protein assay method. US Patent 5,210,017 dated May 11 1993

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