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HOPC 1F/12
HOPC 1F/12
規格:
價格:
編號:B164626
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HOPC 1F/12
商品貨號
B164626
Organism
Mus musculus, mouse
Cell Type
B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
plasmacytoma; myeloma
Strain
BALB/c
Applications
The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma.
The cells are resistant to cortisol.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody,H-2d
Cellular Products
immunoglobulin; monoclonal antibody
Comments
The line was established in culture from the HOPC 1 mineral oil induced BALB/c plasmacytoma.
The cells are resistant to cortisol.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a; lambda 1 light chain
Name of Depositor
M Cohn
Deposited As
Mus musculus
References

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Weigert MG, et al. Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159

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