婷婷91,五月综合在线,久久亚洲色图

Hs 53.T

規格:

價格:

編號:B164689

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

Hs 53.T

商品貨號

B164689

Organism

Homo sapiens, human

Tissue

skin

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

neurofibroma

Age

13 years adolescent

Gender

female

Ethnicity

Black

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 45; range = 34 to 47

Clinical Data

female

Black

13 years adolescent

Comments

Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol:

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.

Subcultivation Ratio: 1:2 to 1:3

Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

STR Profile

Amelogenin: X

CSF1PO: 7,10

D13S317: 9,12

D16S539: 10,12

D5S818: 12,13