久久久久久影视,av一卡二卡,亚洲看片

Hs 746T

規格:

價格:

編號:B164733

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

Hs 746T

商品貨號

B164733

Organism

Homo sapiens, human

Tissue

stomach; derived from metastatic site: left leg

培養基

Cell Type

Epithelial,fibroblast

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

gastric carcinoma

Age

74 years adult

Gender

male

Ethnicity

Caucasian

Applications

Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes.

Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a hypertriploid human cell line having the modal chromosome number of 77 occurring in 46% of cells. Cells with higher ploidies were found at 16.6%. The karyotype of this cell line is very complex. Between 45% and 55% of chromosomes in each cell had structural aberrations, including: der(4)t(1;4) (p13.1;q35); t(15q22q); del(2) (p13); der(7)t(7;?) (p22;?) [7p+]; t(7q22q?) and others. The t(15q22q), del(2), and t(7q22q?) (and several others) had two copies in most cells. There were two types of der(7): one was the 7p+ described above; the other, 7p++, was distinctly longer than N1. There were more than ten markers that were present in some cells only. One DM-like chromosome was found in most cells. Normal N7 and N21 were absent. One normal X and one or two normal Y chromosomes were detected.

Clinical Data

74 years adult

Caucasian

male

Tumorigenic

Yes

Effects

Yes, in immunosuppressed mice

Comments

In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10

D16S539: 11

D5S818: 11,13

D7S820: 8

THO1: 9

TPOX: 8,10

vWA: 15,17

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1-2

PGM3, 1

Name of Depositor

RB Owens

Deposited As

Homo sapiens

Passage History

Year of Origin

1973

References

Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

The cells form carcinomas.

B245006：Conidiobolus lachnodes Drechsler, teleomorph

B244982：Conidiobolus firmipilleus Drechsler, teleomorph

B244898：Phytophthora megasperma Drechsler

B244824：Arthrobotrys brochopaga (Drechsler) Schenck et al., anamorph

B244770：Cochliobolus sativus (Ito et Kuribayashi) Drechsler ex Dastur

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479