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HS-5

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編號:B164788

品牌:Mingzhoubio

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RNA

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凍干粉

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產品名稱

HS-5

商品貨號

B164788

Organism

Homo sapiens, human

Tissue

bone marrow/stroma

培養基

Cell Type

HPV-16 E6/E7 transformed

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

2 [Cells contain human papilloma viral sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

30 years

Gender

male

Ethnicity

Caucasian

Applications

HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.

The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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Derivation

Long term bone marrow cells were transformed with the amphotropic retrovirus vector LXSN16E6E7 in the presence of polybrene. Twenty-seven immortalized clones designated HS-1 to HS-27 were isolated.

Clinical Data

30 years

Caucasian

male

Genes Expressed

granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), macrophage-CSF (M-CSF), Kit ligand (KL), macrophage-inhibitory protein-1 alpha, interleukin-1 alpha (IL-1alpha), IL-1beta, IL-1RA, IL-6, IL-8, IL-11, and leukemia inhibitory factor (LIF)

Comments

One of these cell lines HS-27A (ATCC CRL-2496) has been deposited in the ATCC's general collection. One cell line (HS-5) has been deposited in the Patent Depository (CRL-11882).

HS-5 supports proliferation of hematopoietic progenitor cells when co-cultured in serum-deprived media with no exogenous factors.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:9 is recommended

Medium Renewal: Everh 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Name of Depositor

Fred Hutchinson Cancer Res. Cntr.

Deposited As

human

U.S. Patent Number

5,879,940

References

Roecklein BA, Torok-Storb B. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Blood 85: 997-1005, 1995. PubMed: 7849321

Torok-Storb B, et al. Human marrow stromal cell lines which sustain hematopoieses. US Patent 5,879,940 dated Mar 9 1999

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