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Hs68

規格:

價格:

編號:B164789

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Hs68

商品貨號

B164789

Organism

Homo sapiens, human

Tissue

skin; foreskin

培養基

Cell Type

fibroblast

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

aspartoacylase deficiency; possible Canavan disease

Age

newborn

Gender

male

Ethnicity

Caucasian

Karyotype

This is a human cell line with the normal 46,XY karyotype. The modal chromosome number was 46, with polyploidy at 3.6%. Both X and Y chromosomes can be identified clearly.

Derivation

Hs68 is one of a series of human foreskin fibroblast lines developed at the Naval Biosciences Laboratory (NBL) in Oakland, CA. The material was obtained from an apparently normal Caucasian newborn male in February, 1969.

Clinical Data

Caucasian

male

newborn

Comments

Longevity studies carried out at NBL demonstrated that the cells could be propagated for 42 passages.

The ATCC has been informed by Dr. Anne B. Johnson, Department of Pathology and Neuroscience at Albert Einstein College of Medicine, that this cell line has an unusually low level of the enzyme aspartoacylase (0.028 mU/mg or less on repeat assays.

Our records indicate that the donor of Hs 68 was apparently normal, but Dr. Johnson pointed out that individuals exhibiting a deficiency in aspartoacylase suffer from Canavan disease. Canavan disease is a devastating, autosomal recessive disorder involving spongy degeneration of the nervous system.According to Dr. Johnson, fibroblasts from affected children have aspartoacylase levels of 0.003 to 0.02 mU/mg; carriers' levels vary from 0.016 to 0.04 mU/mg and normal run from 0.1 to 0.4 mU/mg. More information on aspartoacylase and Canavan disease can be found in an article by R. Kaul et al.

Ref

Kaul, P et al. Purification, characterization, and localization of aspartoacylase from bovine brain. J. Neurochem. 56(1): 129-135, 1991. PubMed: 1987315

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 9,12

D13S317: 11,13

D16S539: 11,12

D5S818: 8,11

D7S820: 11,12

THO1: 6,9.3

TPOX: 11

vWA: 16

Name of Depositor

RB Owens

Deposited As

Homo sapiens

Passage History

Longevity studies carried out at NBL demonstrated that the cells could be propagated for 42 passages.

References

Kapatos G. Tetrahydrobiopterin synthesis rate and turnover time in neuronal cultures from embryonic rat mesencephalon and hypothalamus. J. Neurochem. 55: 129-136, 1990. PubMed: 2355214

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Kaul, P et al. Purification, characterization, and localization of aspartoacylase from bovine brain. J. Neurochem. 56(1): 129-135, 1991. PubMed: 1987315

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