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HT-1376
HT-1376
規格:
價格:
編號:B164799
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HT-1376
商品貨號
B164799
Organism
Homo sapiens, human
Tissue
urinary bladder
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
grade 3, carcinoma
Age
58 years
Gender
female
Ethnicity
Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal chromosome number = 104 to 121
Clinical Data
58 years
Caucasian
female
Genes Expressed
fibrinolytic activity; interferon
Cellular Products
fibrinolytic activity; interferon
Tumorigenic
Yes
Effects
Yes, in mice and hamsters
Comments
The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37°C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18
Isoenzymes
G6PD, B
Name of Depositor
S Rasheed
Deposited As
Homo sapiens
References

Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628

Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805

Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

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