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HuNS1

規格:

價格:

編號:B164806

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HuNS1

商品貨號

B164806

Organism

Homo sapiens, human

Cell Type

lymphoblast

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

myeloma

Gender

male

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).

Clinical Data

male

Comments

The cells are HAT sensitive and are negative for Epstein-Barr virus.

According to information provided by the patent depositor (US Patent 4,720,459 dated Jan 19 1988), this cell line did not secrete an immunoglobulin. However, recent testing at ATCC has confirmed the presence of IgG.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%

Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵and 1 x 10⁶cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Complete Culture Medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 12

D16S539: 11

D5S818: 10,12

D7S820: 10

THO1: 9,9.3

TPOX: 8

vWA: 16,19

Name of Depositor

Medical College of Wisconsin Research Foundation, Inc.

Deposited As

Homo sapiens

U.S. Patent Number

4,720,459

References

Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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