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I/24.D6
I/24.D6
規格:
價格:
編號:B164816
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
I/24.D6
商品貨號
B164816
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an epitope encoded by variable exon C of mouse CD45.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD45RC
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD45RC
Comments
Animals were immunized with activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an epitope encoded by variable exon C of mouse CD45.
This epitope is expressed at high levels on essentially all mouse B cells and at lower levels on virtually all CD8 positive (CD8+) splenic T cells.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
1% non-essential amino acids and 50 uM 2-mercaptoethanol, 80%; ORIGEN
(Hybridoma Cloning Factor), 10%; heat-inactivated fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgM
Name of Depositor
G Lazlo, KS Hathcock
Deposited As
rat (B cell); mouse (myeloma)
References

Hathcock KS, et al. Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J. Immunol. 148: 19-28, 1992. PubMed: 1370168

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