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IB4
IB4
規格:
價格:
編號:B164822
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IB4
商品貨號
B164822
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for human CD18, and inhibits CD18 dependent activities.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD18 (leukocyte antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against human CD18 (leukocyte antigen)
Comments
Animals were immunized with human peripheral blood monocytes.
Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.
The antibody is specific for human CD18, and inhibits CD18 dependent activities.
Complete Growth Medium
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a kappa
Name of Depositor
Rockefeller Univ.
U.S. Patent Number
References

Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974

Wright SD, Tuomanen E. Method of inhibiting the influx of leukocytes into organs during sepsis or other trauma. US Patent 5,147,637 dated Sep 15 1992

Wright SD, et al. Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies. Proc. Natl. Acad. Sci. USA 80: 5699-5703, 1983. PubMed: 6225125

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