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IE-10

規格:

價格:

編號:B164826

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

IE-10

商品貨號

B164826

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody specifically binds to mouse ZP3 glycoprotein (amino acids 336-342).

The antibody cross-reacts with rat zonae but not with non-rodent species.

Derivation

Spleen cells were fused with Sp2/0 mouse myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against mouse ZP3 glycoprotein

Cellular Products

immunoglobulin; monoclonal antibody; against mouse ZP3 glycoprotein

Comments

Animals were immunized with particulate murine zonae pellucidae.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

During development and maturation, mammalian oocytes are surrounded by the zona pellucida, which in the mouse is comprised of three sulfated glycoproteins, ZP1, ZP2 and ZP3.

The zona pellucida mediates sperm binding at fertilization, provides a post-fertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct.

The antibody specifically binds to mouse ZP3 glycoprotein (amino acids 336-342).

The antibody cross-reacts with rat zonae but not with non-rodent species.

Following passive immunization, this antibody completely blocks both in vivo and in vitro fertilization of subsequently ovulated mouse eggs.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10 exp4 and 5 X 10 exp5 viable cells/ml.

Attached cells may be subcultured by scraping.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG2a

Name of Depositor

J Dean

Deposited As

rat (B cell); mouse (myeloma)

Passage History

The zona pellucida mediates sperm binding at fertilization, provides a post-fertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct.

References

East IJ, et al. Monoclonal antibodies to the murine zona pellucida protein with sperm receptor activity: effects on fertilization and early development. Dev. Biol. 109: 268-273, 1985. PubMed: 3996750

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Millar SE, et al. Vaccination with a synthetic zona pellucida peptide produces long-term contraception in female mice. Science 246: 935-938, 1989. PubMed: 2479101

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547

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