Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
This is one of a series of hybridomas produced by Dr.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine IgM
Cellular Products
immunoglobulin; monoclonal antibody; against bovine IgM
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bovine IgM.
Spleen cells were fused with P3X63Ag8 myeloma cells.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Complete Growth Medium
RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
